Prevalence of adhesion Virulence factor genes, antibiogram, and pathogenicity of avian Pasteurella multocida isolate from Iran

Authors

  • Ahmad Reza Jabbari Pasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
  • Keyvan Tadayon Department of Aerobic Bacterial Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
Abstract:

Pasteurella multocida possesses various virulence factors, including capsule, lipopolysaccharide, fimbriae, toxins, outer membrane proteins, and adhesions. Adhesins have a crucial role in mediating colonization and invasion of the host. The aim of the present study was to identify the prevalence of adhesion factor genes and resistance/sensitivity patterns among the avian P. multocida isolates from Iran. A total of 30 isolates of P. multocida were used for this study. All isolates were obtained from the poultry cases dead from fowl cholera in the northern parts of Iran. The results of the polymerase chain reaction analysis for the frequency of virulence-associated genes showed that the genes encoding adhesins (i.e., ptfA, fimA, hsf-1, pfhA, and ompH) were found in all (100%) of the isolates. However, the frequency of two genes including tadD and toxA were 50% and 70%, respectively. Thegenotyping patterns were classified into four groups according to the virulence factors in P. multocida isolates. Genotype pattern I, which included the isolates harbouring all of the examined virulence factor genes showed the highest frequency (43.3%). Pathogenicity test showed that all of the isolates classified as genotype I were pathogen or highly pathogen in the mice model. The sensitivity of the isolates to penicillin, ampicillin, lincospectin, florfenicol, tylosin, and tiamulin was 100%. However, the sensitivity rates to flumequin, enrofloxacine, and nalidixicacid were 96.6% and 80%, respectively. The findings of the current study will be helpful to elucidate the disease process and develop an efficient multivalent local vaccine.  

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Distribution of virulence adhesion associated genes and antimicrobial susceptibility in Pasteurella multocida from ovine pasteurellosis in Iran

Pasteurella multocida is an opportunistic pathogen responsible for pneumonia of lambs and goats, respiratory atrophic rhinitis of swine. Although the molecular basis of the pathogenicity and host specificity of P. multocida is not well understood, several studies have reported that a number of proteins are correlated with the pathogenic mechanisms. Adhesins have a crucial role in mediating colo...

full text

distribution of virulence adhesion associated genes and antimicrobial susceptibility in pasteurella multocida from ovine pasteurellosis in iran

pasteurella multocida is an opportunistic pathogen responsible for pneumonia of lambs and goats, respiratory atrophic rhinitis of swine. although the molecular basis of the pathogenicity and host specificity of p. multocida is not well understood, several studies have reported that a number of proteins are correlated with the pathogenic mechanisms. adhesins have a crucial role in mediating colo...

full text

Virulence of Avian Serotype A1 Pasteurella multocida for Chickens and Mice.

The virulence of Pasteurella multocida (P. multocida) serotype A1 for chickens and mice was determined. Groups of chicken and mice were exposed intramuscularly and intraperitoneally to various concentration of P. multocida broth culture, respectively. This strain was highly virulent for chickens so that those exposed to only 7 c.f.u. of the organism died in less than 24 hours. Groups of mice ex...

full text

Isolation, Identification and Antibiogram of Pasteurella Multocida Isolates of Avian Origin

Isolation of Pasteurella multocida was attempted from the heart blood, spleen, liver and lung collected from turkeys (4 No,s) and chicken (4 No,s) suspected to have died of avian pasteurellosis. A total of eight P. multocida isolates were isolated and identified on the basis of biochemical characteristics, pathogenicity studies in mice and PM-PCR. The In vitro antibiotic sensitivity test of the...

full text

Molecular typing of avian Pasteurella multocida isolates from Iran by PCR-RFLP of OmpH gene

Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. All of the P.multocida isolates were recovered from fowl cholera outbreaks in Northern provinces of Iran. Amplification was based on the gene ompH, encoding a major outer membrane protein (protein H). PCR with ompH primers amplified...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 72  issue 2

pages  83- 91

publication date 2017-07-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023